"ECH-Lysine" Related Products

ECH-Lysine Agarose 4 Fast Flow

Cat.no: ECH-Lysine-001A
Product Overview: ECH-Lysine agarose 4 Fast Flow is a group specific medium for isolation of plasminogen and plasminogen activator. It is based on highly crosslinked 4% agarose thus enabling rapid processing of large sample volumes.
Characteristics: It is based on highly crosslinked 4% agarose thus enabling rapid processing of large sample volumes. L-Lysine is covalently bound to a long hydrophilic spacer arm attached to agarose 4 Fast Flow via a stable ether linkage.
Matrix: 4% cross-linked agarose
Average particle size: 90 μm
Ligand: L-lysine
Ligand concentration: 13-18 μmol lysine/ml drained medium
Coupling chemistry: NHS
Dynamic binding capacity: >1.5 mg Plasminogen/ml drained medium
Recommended flow rate: 150-250 cm/h
Recommended column height: 5‐20cm
Chemical stability: The amide bond between the lysine and the spacer arm is stable under alkaline conditions.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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