"Lysine" Related Products

Lysine Agarose

Cat.no: Lysine-001A
Product Overview: Lysine agarose 4B media with immobilized amino acid lysine is mainly used for isolation of plasminogen and plasminogen activator. The ε-amino and α-carboxyl groups on lysine is free after immobilization and interact with a range of biomolecules.
Characteristics: Lysine agarose 4B is L-lysine immobilized to agarose 4B by the cyanogen bromide method. L-lysine is coupled via its α-amino group, leaving both the ε-amino and α-carboxyl groups free to interact with sample substances during chromatography. It is designed for purification of molecules with biospecific or charge dependent affinity for L-lysine. Lysine agarose 4B is a group specific adsorbent and has been used for isolation of plasminogen and plasminogen activator, separation of ribosomal RNA (rRNA) and purification of doublestranded DNA. The precise mechanism by which separation occurs is not fully understood but both electrostatic and stereospecific effects may contribute to the separation, depending upon the application.
Size: 15 g, 250 g
Matrix: Agarose, 4%
Functional group: Lysine
Average particle size: 90 μm
Ligand: L-lysine
Ligand concentration: 4–7 μmol lysine/ml drained medium
Dynamic binding capacity: 0.6 mg human plasminogen/ml drained medium; 0.6–0.7 mg rRNA/ml drained medium
Chemical stability: Stable to all commonly used aqueous buffers
Physical stability: Negligible volume variation due to changes in pH or ionic strength
pH working range: 3-12
pH CIP range: 2-13

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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