"Phenyl" Related Products

Phenyl Cellulose resin

Cat.no: Phenyl-001C
Product Overview: Phenyl Cellulose is useful for the chromatography of hydrophobic proteins. Many proteins have hydrophobic amino acid residues which will interact with the phenyl, butyl and octyl functional groups. Factors that influence this hydrophobic interaction include salt concentration, temperature, pH, organic solvents and surfactants. Protein adsorption usually occurs at high ionic strength, while elution occurs at lower salt concentrations. This is the opposite of ion exchange chromatography and complementary.
Characteristics: 1. Spherical particles exhibiting high mechanical strength 2. Virtually no shrinkage or swelling 3. Stable in organic solvents and surfactants 4. Stable coupling chemistry 5. Resistant to 0.2 M NaOH
Form: suspension in 20 % EtOH
Matrix: cellulose
Functional group: Phenyl group
Average particle size: 40 – 130 μm
Chemical stability: pH 2 – 13, when operated at room temperature. Stable in most salts (NaCl, (NH4)2SO4, etc.) and most detergents (SDS, Tween, Chaps,etc.) Can be cleaned using 0.2 N NaOH.Autoclavable in suspension at neutral pH for 20 minutes at 121℃.
Physical stability: pH 2 – 13, when operated at room temperature. Stable in most salts (NaCl, (NH4)2SO4, etc.) and most detergents (SDS, Tween, Chaps,etc.) Can be cleaned using 0.2 N NaOH.Autoclavable in suspension at neutral pH for 20 minutes at 121℃.
Autoclavable: 121°C for 15 min in distilled water
Shelf life: 5 years from date of manufacture
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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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