"Butyl" Related Products

Butyl Agarose Fast Flow

Cat.no: BUTYL FF-001A
Product Overview: Butyl FF are prepacked Butyl agarose Fast Flow columns for hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elution conditions.
Characteristics: Butyl agarose FF is based on a 90 μm matrix. It is intended for initial and intermediate step purifications requiring amatrix with low to medium hydrophobicity. Butyl agarose FF often works efficiently with rather low salt concentrations.The mechanism of adsorption and desorption onto the butyl ligand is different from that onto the phenyl ligand, giving a differentselectivity.
Form: Slurry in 20% Ethanol
Size: 5 mL
Column: HiTrap
Matrix: 4% crosslinked agarose with spherical beads
Average particle size: 90 μm
Ligand: Butyl
Ligand concentration: 40 μmol/ml medium
Chemical stability: Good chemical stability allows the use of the prepacked columns with most commonly used buffers and organic solvents, like for example 1 M NaOH, 70% ethanol, 30% isopropanol, 6 M GuHCl and 8 M urea. The compatibility with other solvents have been verified by conducting stability test for some of the media with 1 M acetic acid, 3 M (NH4) SO4 and 1 M HCl.
pH working range: 3-13
pH CIP range: 2-14
Temperature Stability: 4‐40 °C
Shelf life: minimum 5 years
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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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