"ATP" Related Products

ATP Agarose

Cat.no: ATP-001A
Product Overview: A ligand commonly used for this ATP Agarose resin is ATP (adenosine-5'-triphosphate) since a very large number of proteins such as kinases, motor proteins, and chaperones bind ATP with high affinity.
Characteristics: ATP Agarose is used in the purification of ATP binding proteins. Any protein with an accessible ATP-binding domain will bind to it, regardless of activation state. The activated proteins will bind to the resin with higher affinity than the corresponding inactivated protein.Better recovery and yields may be obtained when the purification is followed by ion exchange chromatography.
Matrix: Highly cross-linked agarose
Average particle size: 45 - 165 μm
Ligand: ATP
Ligand concentration: 2 – 5 ATP μmol / ml drained gel
Chemical stability: Stable to all solutions commonly used in gel filtration including 8 M urea and 6 M guanidine hydrochloride.Not stable in organic solvents!
pH working range: 7.5
pH CIP range: 4 - 9

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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