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Recombinant Human CTF1 cell lysate

Cat.No. : CTF1-001HCL
Product Overview : Human Cardiotrophin-1 / CTF1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : CTF1 cardiotrophin 1 [ Homo sapiens ]
Official Symbol : CTF1
Synonyms : CTF1; cardiotrophin 1; cardiotrophin-1; CT 1; CT1; cardiophin 1; CT-1;
Gene ID : 1489
mRNA Refseq : NM_001142544
Protein Refseq : NP_001136016
MIM : 600435
UniProt ID : Q16619
Chromosome Location : 16p11.2
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem; MicroRNAs in cardiomyocyte hypertrophy, organism-specific biosystem;
Function : cytokine activity; leukemia inhibitory factor receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does CTF1 contribute to metabolic regulation, particularly in obesity and diabetes? 10/22/2022

CTF1 plays a role in metabolic regulation, impacting processes related to obesity and diabetes.

What are the effects of CTF1 on neuronal survival and regeneration in neurodegenerative diseases? 05/20/2022

CTF1 supports neuronal survival and regeneration, offering potential benefits in neurodegenerative conditions.

How does CTF1 influence cardiac hypertrophy and heart failure progression? 04/22/2022

CTF1 promotes cardiac hypertrophy and can contribute to heart failure progression by influencing heart muscle cell growth.

How do alterations in CTF1 expression affect tissue repair and regeneration after injury? 05/16/2021

Changes in CTF1 expression are important for tissue repair and regeneration, particularly following injury.

What role does CTF1 play in the regulation of muscle regeneration and muscle wasting diseases? 08/05/2020

CTF1 aids in muscle regeneration and can be involved in the pathology of muscle wasting diseases.

How does CTF1 interact with the JAK/STAT signaling pathway in different cell types? 08/25/2019

CTF1 activates the JAK/STAT signaling pathway, affecting cell growth, differentiation, and survival.

What is the impact of CTF1 in inflammatory responses and autoimmune diseases? 08/14/2019

CTF1 influences inflammatory and immune responses, potentially impacting autoimmune disease mechanisms.

Customer Reviews (3)

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Reviews
08/19/2022

    Swift protein characterization services; key for our research projects.

    09/28/2019

      Expertise in protein engineering; invaluable for our lab's needs.

      08/31/2019

        High-quality protein-protein interaction assays; essential for our experiments.

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