Recombinant Human CTF1 cell lysate
Cat.No. : | CTF1-001HCL |
Product Overview : | Human Cardiotrophin-1 / CTF1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer. |
- Specification
- Gene Information
- Related Products
Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | CTF1 cardiotrophin 1 [ Homo sapiens ] |
Official Symbol : | CTF1 |
Synonyms : | CTF1; cardiotrophin 1; cardiotrophin-1; CT 1; CT1; cardiophin 1; CT-1; |
Gene ID : | 1489 |
mRNA Refseq : | NM_001142544 |
Protein Refseq : | NP_001136016 |
MIM : | 600435 |
UniProt ID : | Q16619 |
Chromosome Location : | 16p11.2 |
Pathway : | Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem; MicroRNAs in cardiomyocyte hypertrophy, organism-specific biosystem; |
Function : | cytokine activity; leukemia inhibitory factor receptor binding; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionCTF1 plays a role in metabolic regulation, impacting processes related to obesity and diabetes.
CTF1 supports neuronal survival and regeneration, offering potential benefits in neurodegenerative conditions.
CTF1 promotes cardiac hypertrophy and can contribute to heart failure progression by influencing heart muscle cell growth.
Changes in CTF1 expression are important for tissue repair and regeneration, particularly following injury.
CTF1 aids in muscle regeneration and can be involved in the pathology of muscle wasting diseases.
CTF1 activates the JAK/STAT signaling pathway, affecting cell growth, differentiation, and survival.
CTF1 influences inflammatory and immune responses, potentially impacting autoimmune disease mechanisms.
Customer Reviews (3)
Write a reviewSwift protein characterization services; key for our research projects.
Expertise in protein engineering; invaluable for our lab's needs.
High-quality protein-protein interaction assays; essential for our experiments.
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