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Recombinant H1N1 NA cell lysate

Cat.No. : NA-002H1N1CL
Product Overview : Influenza A H1N1 Neuraminidase / NA ( N295S mutation ) derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : H1N1
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : NA neuraminidase [ Influenza A virus (A/Puerto Rico/8/1934(H1N1)) ]
Official Symbol : NA
Synonyms : neuraminidase
Gene ID : 956530
Protein Refseq : NP_040981
UniProt ID : P03468
Chromosome Location : segment: segment 6
Pathway : Assembly of Viral Components at the Budding Site, organism-specific biosystem; Fusion of the Influenza Virion to the Host Cell Endosome, organism-specific biosystem; Influenza Virus Induced Apoptosis, organism-specific biosystem

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Are there NA protein-based therapies in clinical use? 05/01/2023

Yes, some RNA-based therapies target specific NA proteins for conditions like spinal muscular atrophy.

How do histones relate to epigenetic modifications and clinical outcomes? 08/07/2019

Changes in histone modifications can influence gene expression and contribute to various diseases.

How are NA proteins used in cancer research and treatment? 03/20/2019

NA proteins are studied to understand oncogenesis, and some drugs target NA protein interactions to treat cancer.

Can NA proteins be used for gene therapy? 07/18/2018

NA proteins can be involved in gene therapy strategies to manipulate gene expression for therapeutic purposes.

How are NA proteins involved in the regulation of cell cycle and cancer progression? 12/05/2016

NA proteins control the cell cycle, and dysregulation can contribute to uncontrolled cell growth seen in cancer.

Customer Reviews (3)

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Reviews
11/26/2020

    I highly recommend the NA protein for a variety of experimental applications.

    09/08/2019

      I enthusiastically recommend the NA protein to fellow researchers who seek an unparalleled product accompanied by dedicated customer assistance.

      11/16/2018

        the NA protein stands out for its exceptional quality, coupled with the outstanding technical support delivered by the manufacturer.

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