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Recombinant Human TCN2 cell lysate

Cat.No. : TCN2-001HCL
Product Overview : Human TCN2 / Transcobalamin-II derived in CHO cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : CHO cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : TCN2 transcobalamin II [ Homo sapiens ]
Official Symbol : TCN2
Synonyms : TCN2; transcobalamin II; transcobalamin II; macrocytic anemia; transcobalamin-2; D22S676; D22S750; macrocytic anemia; TC2; vitamin B12-binding protein 2; II; TC; TC-2; TCII; TC II;
Gene ID : 6948
mRNA Refseq : NM_000355
Protein Refseq : NP_000346
MIM : 613441
UniProt ID : P20062
Chromosome Location : 22q12.2
Pathway : One Carbon Metabolism, organism-specific biosystem; Vitamin digestion and absorption, organism-specific biosystem; Vitamin digestion and absorption, conserved biosystem;
Function : cobalamin binding; cobalamin binding; cobalamin binding; metal ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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01/30/2023

    Cheap price, high quality products, worth buying again.

    08/16/2022

      The TCN2 proteins purchased from your company is suitable for ELISA.

      07/21/2021

        The technical expert will follow up on the progress of my experiments and provide constructive advice on the problems that arise in my experiments.

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