"TPP1" Related Products


Recombinant Human TPP1 cell lysate

Cat.No.: TPP1-449HCL
Product Overview: Human TPP1 / CLN2 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
Source: Baculovirus-Insect Cells
Species: Human
Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability: Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name: TPP1 tripeptidyl peptidase I [ Homo sapiens ]
Official Symbol: TPP1
Synonyms: TPP1; tripeptidyl peptidase I; ceroid lipofuscinosis, neuronal 2, late infantile (Jansky Bielschowsky disease) , CLN2; tripeptidyl-peptidase 1; TPP I; tripeptidyl aminopeptidase; growth-inhibiting protein 1; cell growth-inhibiting gene 1 protein; lysosomal pepstatin insensitive protease; CLN2; GIG1; LPIC; TPP-1; MGC21297;
Gene ID: 1200
mRNA Refseq: NM_000391
Protein Refseq: NP_000382
MIM: 607998
UniProt ID: O14773
Chromosome Location: 11p15.4
Pathway: Activation of Chaperone Genes by XBP1(S), organism-specific biosystem; Activation of Chaperones by IRE1alpha, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Unfolded Protein Response, organism-specific biosystem;
Function: endopeptidase activity; endopeptidase activity; metal ion binding; peptidase activity; peptide binding; protein binding; serine-type endopeptidase activity; serine-type peptidase activity; tripeptidyl-peptidase activity; tripeptidyl-peptidase activity;

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