Recombinant Human GAD1 cell lysate
Cat.No. : | GAD1-586HCL |
Product Overview : | Human GAD1 / Glutamate decarboxylase 1 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | GAD1 glutamate decarboxylase 1 (brain, 67kDa) [ Homo sapiens ] |
Official Symbol : | GAD1 |
Synonyms : | GAD1; glutamate decarboxylase 1 (brain, 67kDa); GAD, glutamate decarboxylase 1 (brain, 67kD); glutamate decarboxylase 1; GAD-67; 67 kDa glutamic acid decarboxylase; glutamate decarboxylase 67 kDa isoform; GAD; SCP; CPSQ1; FLJ45882; |
Gene ID : | 2571 |
mRNA Refseq : | NM_000817 |
Protein Refseq : | NP_000808 |
MIM : | 605363 |
UniProt ID : | Q99259 |
Chromosome Location : | 2q31 |
Pathway : | Alanine and aspartate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Biogenic Amine Synthesis, organism-specific biosystem; Butanoate metabolism, organism-specific biosystem; Butanoate metabolism, conserved biosystem; GABA (gamma-Aminobutyrate) shunt, organism-specific biosystem; |
Function : | carboxy-lyase activity; glutamate decarboxylase activity; lyase activity; protein binding; pyridoxal phosphate binding; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionMolecular techniques such as PCR (Polymerase Chain Reaction) and genetic sequencing are employed to investigate mutations and variations in the GAD1 gene.
Altered GAD1 function can disrupt the balance of GABAergic neurotransmission, potentially contributing to the development or exacerbation of anxiety disorders.
Investigating GAD1 as a therapeutic target may offer novel approaches to modulate GABAergic signaling and manage conditions associated with its dysregulation.
In some cases, alterations in GAD1 expression levels have been proposed as potential biomarkers for specific neurological and psychiatric disorders.
Yes, genetic polymorphisms in the GAD1 gene have been identified, and variations may influence an individual's susceptibility to certain neurological and psychiatric disorders.
Customer Reviews (3)
Write a reviewOne advantage of using GAD1 protein in trials is its versatility.
GAD1 is a key regulator of cellular structure and function, particularly in the context of maintaining the integrity of the cell membrane and organizing cytoskeletal components.
GAD1 can be used in various experimental setups and assays, including in vitro cell culture studies, animal models, and even clinical investigations.
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