Recombinant Human FAM171B cell lysate FAM171B-783HCL

Recombinant Human FAM171B cell lysate

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Recombinant Human FAM171B cell lysate

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Cat.No. : FAM171B-783HCL
Product Overview : Human FAM171B / KIAA1946 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : FAM171B family with sequence similarity 171, member B [ Homo sapiens ]
Official Symbol : FAM171B
Synonyms : KIAA1946
Gene ID : 165215
mRNA Refseq : NM_177454.3
Protein Refseq : NP_803237.3
UniProt ID : Q6P995
Chromosome Location : 2q32.1
Function : DNA binding;

Products Types

◆ Recombinant Protein

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FAM171B-575HRecombinant Human FAM171B Protein, Fc-tagged+Inquiry

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◆ Lysates

FAM171B-001HCLRecombinant Human FAM171B cell lysate+Inquiry

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FAM19A2FAM3CFAM129AFAM129BFAM189A2FAM200AFAM50AFAM65BFAM84AFAM84BFAM127CFAM40AFAM3BFAM3DFAM62BFAM175AFAM20CFAM164CFAM129CFAM203AFAM207AFAM188AFAM110BFAM189BFAM163AFAM206AFAM164AFAM127AFAM32AFAM35AFAM36AFAM107AFAM108A1FAM108B1FAM109AFAM109BFAM111AFAM122BFAM13BFAM13CFAM170AFAM171A1FAM173BFAM175BFAM78BFAM81BFAM101AFAM102AFAM102BFAM104BFAM178BFAM180AFAM181BFAM185AFAM188B2FAM22GFAM26DFAM26FFAM59AFAM5CFAM134BFAM135BFAM136AFAM161AFAM161BFAM19A4FAM21CFAM40BFAM83DFAM155AFAM156AFAM160B1FAM89BFAM105AFAM123BFAM124AFAM125BFAM126AFAM18B1FAM190AFAM194BFAM196AFAM46AFAM46CFAM46DFAM47AFAM47BFAM50BFAM53AFAM53BFAM53CFAM54AFAM54BFAM78AFAM82A2FAM82BFAM83EFAM83FFAM20AFAM166AFAM167AFAM168AFAM168BFAM169AFAM198BFAM111BFAM117AFAM118AFAM118BFAM120AFAM120AOSFAM120CFAM122AFAM150AFAM151AFAM153BFAM154AFAM70AFAM71AFAM71BFAM71DFAM90A1FAM91A1FAM92A1FAM92BFAM96BFAM98AFAM98BFAM174BFAM184BFAM192AFAM114A1FAM132AFAM160B2FAM172AFAM134CFAM76BFAM81AFAM131CFAM116AFAM19A3FAM76AFAM59BFAM188BFAM71F1FAM73AFAM82A1FAM83BFAM83GFAM96AFAM98CFAM110DFAM189A1FAM190BFAM214AFAM176AFAM49AFAM213AFAM204AFAM222AFAM216BFAM211AFAM212BFAM213BFAM209AFAM217BFAM176CFAM211BFAM212AFAM217AFAM221BFAM205AFAM221AFAM75E1FAM199XFAM100BFAM103A1FAM107BFAM104AFAM110CFAM115CFAM116BFAM117BFAM123AFAM125AFAM45AFAM48AFAM49BFAM123CFAM126BFAM131BFAM131AFAM134AFAM135AFAM149B1FAM13AFAM151BFAM154BFAM160A1FAM159BFAM162BFAM162AFAM166BFAM167BFAM171BFAM171A2FAM174AFAM173AFAM178AFAM179AFAM181AFAM180BFAM184AFAM186AFAM186BFAM187BFAM20BFAM19A1FAM26EFAM63AFAM57BFAM65AFAM69AFAM70BFAM71CFAM71F2FAM73BFAM105BFam108aFam115eFAM120BFAM122CFAM132BFAM133BFAM159AFAM160A2FAM163BFAM164BFAM165BFAM176BFam177aFAM177BFAM179BFAM183AFAM183BFAM193AFAM193BFAM194AFAM195AFAM195BFAM196BFAM208AFAM208BFAM210AFAM210BFAM220AFAM24AFAM25AFAM25CFAM38AFAM38BFAM55BFAM57AFAM58BFAM69BFAM69CFAM71E1FAM71E2FAM72AFAM83AFAM83CFAM89AFam92aFAM55CFAM55DFAM75D4FAM33AFAM23AFam100aFam101bFam108bFam108c1Fam110aFam114a2Fam124bFAM127BFAM133AFAM149AFam149bFam150bFAM155BFAM156BFAM157AFAM157BFam169bFam170bFAM177A1Fam18aFam18bFam18b2Fam187aFAM19A5FAM198AFAM200BFAM209BFAM21AFAM21BFAM214BFAM218AFAM219AFAM22AFAM22DFAM22FFAM222BFAM228AFAM25BFAM25GFam3aFam43aFam43bFam46bFam47cFam47eFAM48B1FAM48B2Fam5bFAM58AFam60aFam63bFAM64AFam65cFAM72BFAM72DFAM8A1Fam83hFam86aFAM86B1FAM86B2FAM86C1Fam86FAM9AFAM75A1FAM75A2FAM75A3FAM75A5FAM75A6FAM75A7FAM75C1FAM75C2FAM75D3Fam75d1aFam75d1bFam75d1cFam75d1dFam75aFAM115A

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