Recombinant Human AMIGO2 cell lysate
Cat.No. : | AMIGO2-1147HCL |
Product Overview : | Human AMIGO2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
- Related Products
Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | AMIGO2 adhesion molecule with Ig-like domain 2 [ Homo sapiens ] |
Official Symbol : | AMIGO2 |
Synonyms : | AMIGO2; adhesion molecule with Ig-like domain 2; amphoterin-induced protein 2; ALI1; amphoterin induced gene and open reading frame 2; DEGA; alivin 1; amphoterin induced gene 2; transmembrane protein AMIGO2; differentially expressed in gastric adenocarcinoma; differentially expressed in gastric adenocarcinomas; AMIGO-2; |
Gene ID : | 347902 |
mRNA Refseq : | NM_181847 |
Protein Refseq : | NP_862830 |
UniProt ID : | Q86SJ2 |
Chromosome Location : | 12q13.11 |
Products Types
◆ Recombinant Protein | ||
AMIGO2-309R | Recombinant Rat AMIGO2 Protein, His (Fc)-Avi-tagged | +Inquiry |
AMIGO2-385H | Recombinant Human AMIGO2 Protein, His-tagged | +Inquiry |
AMIGO2-568HB | Recombinant Human AMIGO2 protein, His-tagged, Biotinylated | +Inquiry |
AMIGO2-0019H | Recombinant Human AMIGO2 Protein (Val40-His393), C-His-tagged | +Inquiry |
AMIGO2-0018H | Recombinant Human AMIGO2 Protein (Thr30-Ala399), N-His-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (10)
Ask a questionAltered expression or dysregulation of AMIGO2 has been associated with neurological disorders, including autism spectrum disorders and epilepsy, highlighting its potential role in disease pathogenesis.
AMIGO2 exhibits a dynamic expression pattern in the developing and adult brain, with high expression in regions involved in neuronal development, such as the cortex, hippocampus, and cerebellum.
Knockout or overexpression studies of AMIGO2 have revealed its essential role in neuronal development and function, impacting processes such as neurite outgrowth, synaptogenesis, and neuronal survival.
AMIGO2 is involved in modulating synaptic plasticity and neurotransmission, regulating the activity and surface expression of neurotransmitter receptors and contributing to the refinement of synaptic connectivity.
AMIGO2 influences dendritic arborization and synaptic connectivity, promoting dendritic branching and the formation of mature dendritic spines, which are crucial for synaptic transmission and plasticity.
AMIGO2 plays a role in myelination and oligodendrocyte development, contributing to proper myelin sheath formation and the maturation of oligodendrocytes.
Modulation of AMIGO2 expression or activity holds potential as a therapeutic approach for neurological conditions, and further investigation into its specific mechanisms and interactions may unveil novel strategies for intervention and treatment.
AMIGO2 is involved in axon guidance and pathfinding, guiding axons to their appropriate targets during neuronal development, and contributing to the establishment of neuronal circuitry.
AMIGO2 plays a critical role in neuronal migration during brain development, regulating the proper positioning and layering of neurons in the developing cerebral cortex.
AMIGO2 interacts with various cell adhesion molecules and signaling proteins, including members of the immunoglobulin superfamily and growth factor receptors, activating downstream signaling pathways involved in cell adhesion, cytoskeletal remodeling, and synaptic plasticity.
Customer Reviews (3)
Write a reviewVersatility in multiple assay formats and platforms.
Excellent reproducibility in protein expression profiling.
Stable reagents for long-term storage and repeated use.
Ask a Question for All AMIGO2 Products
Required fields are marked with *
My Review for All AMIGO2 Products
Required fields are marked with *
Inquiry Basket