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Recombinant Human AMIGO2 cell lysate

Cat.No. : AMIGO2-1147HCL
Product Overview : Human AMIGO2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : AMIGO2 adhesion molecule with Ig-like domain 2 [ Homo sapiens ]
Official Symbol : AMIGO2
Synonyms : AMIGO2; adhesion molecule with Ig-like domain 2; amphoterin-induced protein 2; ALI1; amphoterin induced gene and open reading frame 2; DEGA; alivin 1; amphoterin induced gene 2; transmembrane protein AMIGO2; differentially expressed in gastric adenocarcinoma; differentially expressed in gastric adenocarcinomas; AMIGO-2;
Gene ID : 347902
mRNA Refseq : NM_181847
Protein Refseq : NP_862830
UniProt ID : Q86SJ2
Chromosome Location : 12q13.11

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Is there a correlation between AMIGO2 expression levels and neurological disorders? 03/25/2022

Altered expression or dysregulation of AMIGO2 has been associated with neurological disorders, including autism spectrum disorders and epilepsy, highlighting its potential role in disease pathogenesis.

What is the expression pattern of AMIGO2 in the developing and adult brain? 12/13/2021

AMIGO2 exhibits a dynamic expression pattern in the developing and adult brain, with high expression in regions involved in neuronal development, such as the cortex, hippocampus, and cerebellum.

What are the functional consequences of AMIGO2 knockout or overexpression in neuronal development and function? 11/07/2021

Knockout or overexpression studies of AMIGO2 have revealed its essential role in neuronal development and function, impacting processes such as neurite outgrowth, synaptogenesis, and neuronal survival.

Can AMIGO2 modulate synaptic plasticity and neurotransmission? 03/11/2021

AMIGO2 is involved in modulating synaptic plasticity and neurotransmission, regulating the activity and surface expression of neurotransmitter receptors and contributing to the refinement of synaptic connectivity.

How does AMIGO2 influence dendritic arborization and synaptic connectivity? 09/19/2020

AMIGO2 influences dendritic arborization and synaptic connectivity, promoting dendritic branching and the formation of mature dendritic spines, which are crucial for synaptic transmission and plasticity.

Does AMIGO2 play a role in myelination and oligodendrocyte development? 09/11/2020

AMIGO2 plays a role in myelination and oligodendrocyte development, contributing to proper myelin sheath formation and the maturation of oligodendrocytes.

Can manipulation of AMIGO2 expression or activity be a potential therapeutic approach for neurological conditions? 09/07/2020

Modulation of AMIGO2 expression or activity holds potential as a therapeutic approach for neurological conditions, and further investigation into its specific mechanisms and interactions may unveil novel strategies for intervention and treatment.

What is the role of AMIGO2 in axon guidance and pathfinding? 12/27/2017

AMIGO2 is involved in axon guidance and pathfinding, guiding axons to their appropriate targets during neuronal development, and contributing to the establishment of neuronal circuitry.

How does AMIGO2 contribute to neuronal migration during brain development? 11/08/2017

AMIGO2 plays a critical role in neuronal migration during brain development, regulating the proper positioning and layering of neurons in the developing cerebral cortex.

Does AMIGO2 interact with other cell adhesion molecules or signaling proteins, and what are the downstream signaling pathways involved? 10/06/2017

AMIGO2 interacts with various cell adhesion molecules and signaling proteins, including members of the immunoglobulin superfamily and growth factor receptors, activating downstream signaling pathways involved in cell adhesion, cytoskeletal remodeling, and synaptic plasticity.

Customer Reviews (3)

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Reviews
05/17/2021

    Versatility in multiple assay formats and platforms.

    09/24/2020

      Excellent reproducibility in protein expression profiling.

      11/02/2017

        Stable reagents for long-term storage and repeated use.

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