Recombinant Cynomolgus CD3D & CD3E cell lysate


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Recombinant Cynomolgus CD3D & CD3E cell lysate

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Cat.No. : CD3D & CD3E-1179CCL
Product Overview : Cynomolgus CD3D & CD3E Heterodimer derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
Source : Human cells
Species : Cynomolgus
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : CD3D CD3d molecule, delta (CD3-TCR complex) [ Macaca fascicularis ]; CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Macaca fascicularis ]
Official Symbol : CD3D; CD3E
Synonyms : T-cell surface glycoprotein CD3 delta chain; CD3 delta; T-cell receptor T3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; CD3 epsilon FN18+; CD3 epsilon FN18-
Gene ID : 102133701; 102133065
mRNA Refseq : NM_001287688; NM_001283615
Protein Refseq : NP_001274617; NP_001270544
UniProt ID : Q95LI8; Q95LI5
Chromosome Location : chromosome: 14; chromosome: 14
Pathway : Chagas disease (American trypanosomiasis), organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem. Chagas disease (American trypanosomiasis), organism-specific biosystem; HTLV-I infection, organism-specific biosystem; Primary immunodeficiency, conserved biosystem
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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