Recombinant Rat ERBB3 cell lysate
Cat.No. : | ERBB3-1415RCL |
Product Overview : | Rat ErbB3 / HER3 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Rat |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Erbb3 v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) [ Rattus norvegicus ] |
Official Symbol : | ERBB3 |
Synonyms : | ERBB3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian); receptor tyrosine-protein kinase erbB-3; c-erbB3; c-erbB-3; avian erythroblastosis oncogene B 3; proto-oncogene-like protein c-ErbB-3; |
Gene ID : | 29496 |
mRNA Refseq : | NM_017218 |
Protein Refseq : | NP_058914 |
Pathway : | Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; ErbB signaling pathway, organism-specific biosystem; ErbB signaling pathway, organism-specific biosystem; ErbB signaling pathway, conserved biosystem; |
Function : | ATP binding; growth factor binding; growth factor binding; nucleotide binding; protein heterodimerization activity; protein heterodimerization activity; protein tyrosine kinase activator activity; protein tyrosine kinase activator activity; NOT protein tyrosine kinase activity; receptor activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; |
Products Types
◆ Recombinant Protein | ||
Erbb3-7437R | Recombinant Rat Erbb3 Protein(Met1-His641), His-tagged | +Inquiry |
ERBB3-237H | Recombinant Human ERBB3 Protein, Fc-tagged | +Inquiry |
ERBB3-209H | Active Recombinant Human ERBB3(Met1-Thr643), His-tagged | +Inquiry |
Erbb3-384M | Recombinant Mouse Erbb3 Protein, His-tagged | +Inquiry |
Erbb3-970M | Recombinant Mouse Erbb3 Protein, MYC/DDK-tagged | +Inquiry |
◆ Lysates | ||
ERBB3-430HCL | Recombinant Human ERBB3 cell lysate | +Inquiry |
ERBB3-1765MCL | Recombinant Mouse ERBB3 cell lysate | +Inquiry |
ERBB3-1721RCL | Recombinant Rhesus ERBB3 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionPotential side effects may include skin rash, diarrhea, and cardiovascular issues, as seen with other drugs targeting the EGFR family.
ERBB3 activation has been associated with resistance to some targeted therapies, such as EGFR inhibitors, making it a challenging target in cancer treatment.
ERBB3 can form heterodimers with other EGFR family members, such as EGFR and HER2, leading to the activation of downstream signaling pathways.
Yes, elevated ERBB3 expression has been linked to poor prognosis in certain cancers, serving as a potential biomarker for patient outcomes.
ERBB3 overexpression is frequently observed in breast, ovarian, and gastric cancers, among others.
Customer Reviews (3)
Write a reviewTheir expert team has been instrumental in addressing any challenges or concerns I have encountered during my experiments.
Their prompt and comprehensive assistance has proven invaluable in overcoming obstacles and optimizing my experimental protocols.
The ERBB3 protein boasts remarkable stability, allowing for extended storage without compromising its integrity or performance.
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