Recombinant Mouse CD24 cell lysate CD24-1422MCL

Recombinant Mouse CD24 cell lysate

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Recombinant Mouse CD24 cell lysate

Cd24a Related Products

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Cat.No. : CD24-1422MCL
Product Overview : Mouse CD24 / Ly-52 / CD24A derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Cd24a CD24a antigen [ Mus musculus ]
Official Symbol : Cd24a
Synonyms : HSA; Cd24; Ly-52; nectadrin; signal transducer CD24; R13-Ag; heat stable antigen; lymphocyte antigen 52; X62 heat stable antigen; cluster of differentiation 24; M1/69-J11D heat stable antigen
Gene ID : 12484
mRNA Refseq : NM_009846
Protein Refseq : NP_033976
UniProt ID : P24807
Chromosome Location : 10 B2; 10 23.01 cM
Pathway : Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem

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