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Recombinant Rat FCGRT & B2M cell lysate

Cat.No. : FCGRT & B2M-1535RCL
Product Overview : Rat FCGRT & B2M Heterodimer derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Rat
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Fcgrt Fc fragment of IgG, receptor, transporter, alpha [ Rattus norvegicus ]; B2m beta-2 microglobulin [ Rattus norvegicus ]
Official Symbol : Fcgrt; B2m
Synonyms : FcRn; beta-2-microglobulin; beta-2-microglobulin C-terminal fragment (55 AA); IgG receptor FcRn large subunit p51; neonatal Fc receptor; Fc fragment immunoglobulin G receptor; Fc receptor, IgG, alpha chain transporter; igG Fc fragment receptor transporter alpha chain
Gene ID : 29558; 24223
mRNA Refseq : NM_03335; NM_012512
Protein Refseq : NP_203502; NP_036644
UniProt ID : P13599; P07151
Chromosome Location : 1q22; 3q35
Pathway : Adaptive Immune System, organism-specific biosystem; Antigen Presentation: Folding, assembly and peptide loading of class I MHC, organism-specific biosystem; Disease, organism-specific biosystem
Function : IgG binding; antigen binding; identical protein binding; molecular_function

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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10/16/2019

    With a long shelf life, a single bottle of the reagent can be used multiple times, which is especially valuable for budget-conscious laboratories.

    07/24/2019

      It maintains its activity and effectiveness under various environmental conditions.

      04/11/2017

        The reagent has a wide range of applications, catering to diverse needs in different laboratories, be it in biomedical research or the field of bioengineering.

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