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Recombinant Mouse ART4 cell lysate

Cat.No. : ART4-1541MCL
Product Overview : Mouse Art4 / CD297 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Art4 ADP-ribosyltransferase 4 [ Mus musculus ]
Official Symbol : ART4
Synonyms : ART4; ADP-ribosyltransferase 4;
Gene ID : 11874

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Does ART4 have any non-enzymatic functions or interactions with other proteins? 01/13/2023

Beyond its enzymatic role in ADP-ribosylation, ART4 does not have well-characterized non-enzymatic functions or specific protein interactions.

How does ART4 catalyze ADP-ribosylation? 05/18/2021

ART4 transfers ADP-ribose from NAD+ to specific target proteins, modifying their structure and function.

Are there any known diseases or disorders associated with mutations in the ART4 gene? 12/19/2019

Mutations in the ART4 gene are not commonly associated with diseases or disorders in humans.

Are there any therapeutic applications targeting ART4? 06/21/2019

ART4 itself is not targeted therapeutically. However, understanding its role in blood group antigen regulation may have implications in blood transfusion medicine and compatibility testing.

What are some known substrates of ART4? 11/26/2018

ART4 ADP-ribosylates specific glycoproteins on the surface of red blood cells, such as the P1 and P2 antigens.

Is the activity of ART4 influenced by any cofactors or regulators? 05/27/2017

ART4 activity depends on the availability of NAD+ as a substrate, and it may also be regulated by cellular signaling pathways related to red blood cell function.

Customer Reviews (3)

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Reviews
01/04/2023

    With its remarkable purity and reliability, coupled with the manufacturer's excellent technical support, this protein is a crucial asset in addressing my scientific inquiries and advancing my research goals.

    06/17/2017

      In addition to its outstanding quality, the Art4 Protein exhibits exceptional stability during storage and usage.

      04/07/2016

        Their team of experts has been instrumental in addressing any issues or concerns I have encountered during my experiments.

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