"PCMT1" Related Products

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Recombinant Human PCMT1, His-tagged

Cat. No.: PCMT1-704H
Product Overview: Recombinant human PCMT1 protien, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 28.8 kDa (263aa).
Description: PCMT1, also known as L-isoaspartyl protein carboxyl methyltransferase, is an enzyme that catalyses the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. This protein plays a role in the repair and/or degradation of damaged proteins.
Source: E.coli.
Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl.
Purity: > 95% by SDS – PAGE.
Concentration: 1 mg/ml (determined by Bradford assay).
Sequences Of Amino Acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAWK SGGASHSELI HNLRKNGIIK TDKVFEVMLA TDRSHYAKCN PYMDSPQSIG FQATISAPHM HAYALELLFD QLHEGAKALD VGSGSGILTA CFARMVGCTG KVIGIDHIKE LVDDSINNVR KDDPTLLSSG RVQLVVGDGR MGYAEEAPYD AIHVGAAAPV VPQALIDQLK PGGRLILPVG PAGGNQMLEQ YDKLQDGSIK MKPLMGVIYV PLTDKEKQWS RWK.
Storage: can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: PCMT1 protein-L-isoaspartate (D-aspartate) O-methyltransferase [ Homo sapiens ]
Synonyms: protein-L-isoaspartate (D-aspartate) O-methyltransferase; PCMT1; protein-L-isoaspartate(D-aspartate) O-methyltransferase; PIMT; OTTHUMP00000017397; OTTHUMP00000017398; OTTHUMP00000017400; protein-beta-aspartate methyltransferase; L-isoaspartyl/D-aspartyl methyltransferase; L-isoaspartyl protein carboxyl methyltransferase; protein L-isoaspartyl/D-aspartyl methyltransferase;protein-L-isoaspartate (D-aspartate) O-methyltransferase 1; Protein-beta-aspartate methyltransferase; Protein L-isoaspartyl/D-aspartyl methyltransferase; EC 2.1.1.77
Gene ID: 5110
mRNA Refseq: NM_005389
Protein Refseq: NP_005380
MIM: 176851
UniProt ID: P22061
Chromosome Location: 6q24-q25
Function: identical protein binding; methyltransferase activity; protein binding; transferase activity; protein-L-isoaspartate (D-aspartate) O-methyltransferase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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