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Recombinant H1N1 HA cell lysate

Cat.No. : HA-2362HCL
Product Overview : Influenza A H1N1 (A/WSN/1933) Hemagglutinin derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : H1N1
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : HA haemagglutinin [ Influenza A virus (A/Puerto Rico/8/1934(H1N1)) ]
Official Symbol : HA
Synonyms : hemagglutinin; HA1; HA2
Gene ID : 956529
Protein Refseq : NP_040980
UniProt ID : P03452
Chromosome Location : segment: segment 4
Pathway : Assembly of Viral Components at the Budding Site, organism-specific biosystem; Budding, organism-specific biosystem; Disease, organism-specific biosystem

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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How about the structural characteristics of HA protein? 09/14/2019

HA protein is a glycoprotein that contains thousands of carbohydrates and an amino acid chain consisting of 566 amino acid residues. The structure of HA protein has triple symmetry, consisting of three monomers that form a spiral.

Will genetic variants in HA proteins affect vaccine development? 06/06/2019

Genetic variants in the HA protein may affect the development and production of influenza vaccines. Because HA protein is an important component of influenza viruses, different subtypes or variants of HA protein may require different vaccines to prepare.

Why is hemagglutinin protein related to viral replication? 05/17/2019

This protein is one of the key factors in the replication process of influenza viruses. When a virus infects a host cell, the receptor-binding region of the HA protein binds to the receptor on the cell surface, allowing the virus to invade the host cell and replicate.

What are the subtypes of HA protein? 04/01/2019

There are currently 18 known HA subtypes of influenza viruses, H1-H18. Among them, the H1, H2, H3, H5, H7, and H9 subtypes are associated with human viruses, while other subtypes are generally associated with animal viruses.

What are the physiological functions of HA protein? 03/07/2019

The main physiological function of HA protein is to bind to host cells and mediate the entry of influenza viruses into host cells. It contains specific regions that bind glycoprotein receptors that allow the influenza virus to adhere to them. This is an important link that allows the virus to stably bind and enter the cell.

What laboratory techniques are HA proteins used in? 01/07/2019

HA protein is widely used in viral research and drug development. Researchers can use HA proteins to test drugs such as influenza virus activators, antibodies and vaccines, and HA proteins are also widely used to develop novel immunoassays and treatments.

Customer Reviews (2)

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Reviews
06/01/2021

    The transport process does not affect the protein quality, and the stability is good.

    11/27/2019

      Higher solubility makes it easy to prepare the desired concentration.

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