Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Mouse CTSA cell lysate

Cat.No. : CTSA-2399MCL
Product Overview : Mouse CTSA / Cathepsin-A derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
  • Related Products
Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Ctsa cathepsin A [ Mus musculus ]
Official Symbol : CTSA
Synonyms : CTSA; cathepsin A; lysosomal protective protein; carboxypeptidase C; carboxypeptidase L; protective protein cathepsin A; protective protein for beta-galactosidase; PPCA; Ppgb; AU019505;
Gene ID : 19025
mRNA Refseq : NM_001038492
Protein Refseq : NP_001033581
Pathway : Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Renin-angiotensin system, organism-specific biosystem; Renin-angiotensin system, conserved biosystem;
Function : carboxypeptidase activity; hydrolase activity; peptidase activity; protein binding; serine-type carboxypeptidase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
Are there any post-translational modifications that regulate CTSA activity? 06/12/2022

Post-translational modifications of CTSA are not well-documented, requiring further research for comprehensive understanding.

How does CTSA influence cellular processes outside the lysosome? 04/04/2022

CTSA has roles in various cellular processes outside the lysosome, though these are less well understood compared to its lysosomal functions.

What is the significance of CTSA in the degradation of glycoproteins? 03/24/2022

CTSA is crucial for the catabolism of glycoproteins, aiding in the removal of terminal sugar moieties.

What therapeutic strategies are being explored to address deficiencies or dysfunctions in CTSA? 01/27/2022

Therapeutic strategies for CTSA-related disorders are in the early stages of development, with enzyme replacement therapy being a potential avenue.

How is CTSA dysfunction associated with Galactosialidosis? 04/28/2020

Dysfunction or deficiency in CTSA leads to Galactosialidosis, characterized by the accumulation of sialyloligosaccharides and glycoproteins in tissues.

How does CTSA contribute to the stability and activation of other lysosomal enzymes? 10/29/2019

It forms a complex with β-galactosidase and neuraminidase, preventing their premature degradation and facilitating their activation.

What is the primary function of CTSA in the lysosome? 10/07/2019

CTSA acts as a protective protein, ensuring the stability and proper function of other lysosomal enzymes.

Customer Reviews (3)

Write a review
Reviews
06/12/2022

    Product arrived quickly, undamaged.

    04/04/2022

      Great results, worth buying.

      01/27/2022

        Highly pure, excellent product.

        Ask a Question for All CTSA Products

        Required fields are marked with *

        My Review for All CTSA Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends