|Product Overview :||Recombinant human IGFBP1 protein (Ala26-Asn259) without tag was expressed in mouse myeloma cell line.|
|Description :||This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP N-terminal domain and a thyroglobulin type-I domain. The encoded protein, mainly expressed in the liver, circulates in the plasma and binds both insulin-like growth factors (IGFs) I and II, prolonging their half-lives and altering their interaction with cell surface receptors. This protein is important in cell migration and metabolism. Low levels of this protein may be associated with impaired glucose tolerance, vascular disease and hypertension in human patients.|
|Bio-activity :||Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. The ED50 for this effect is typically 0.1-0.6 μg/mL in the presence of 6 ng/mL recombinant human IGF-I.|
|Molecular Mass :||25 kDa|
|N-terminal Sequence Analysis :||Ala26|
|Endotoxin :||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity :||>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
|Storage :||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Storage Buffer :||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution :||Reconstitute at 100 μg/mL in sterile PBS.|
|Gene Name :||IGFBP1 insulin like growth factor binding protein 1 [ Homo sapiens (human) ]|
|Official Symbol :||IGFBP1|
|Synonyms :||alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; binding protein-25; binding protein-26; binding protein-28; growth hormone independent-binding protein; hIGFBP-1; IBP1; IBP-1; IGF-binding protein 1; IGFBP1; IGFBP-1; IGF-BP25; insulin-like growth factor binding protein 1; insulin-like growth factor-binding protein 1; Placental protein 12; PP12AFBP|
|Gene ID :||3484|
|mRNA Refseq :||NM_000596|
|Protein Refseq :||NP_000587|
|UniProt ID :||P08833|