Recombinant Human LRPAP1 cell lysate
Cat.No. : | LRPAP1-2574HCL |
Product Overview : | Human LRPAP1 / A2MRAP derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | LRPAP1 low density lipoprotein receptor-related protein associated protein 1 [ Homo sapiens ] |
Official Symbol : | LRPAP1 |
Synonyms : | LRPAP1; low density lipoprotein receptor-related protein associated protein 1; A2MRAP, low density lipoprotein related protein associated protein 1 (alpha 2 macroglobulin receptor associated protein 1); alpha-2-macroglobulin receptor-associated protein; HBP44; alpha-2-MRAP; lipoprotein receptor associated protein; alpha-2-macroglobulin receptor-associated protein 1; low density lipoprotein receptor-related protein-associated protein 1; low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1); RAP; MRAP; A2RAP; A2MRAP; MGC138272; |
Gene ID : | 4043 |
mRNA Refseq : | NM_002337 |
Protein Refseq : | NP_002328 |
MIM : | 104225 |
UniProt ID : | P30533 |
Chromosome Location : | 4p16.3 |
Pathway : | Lissencephaly gene (LIS1) in neuronal migration and development, organism-specific biosystem; Reelin signaling pathway, organism-specific biosystem; Signaling events mediated by the Hedgehog family, organism-specific biosystem; |
Function : | asialoglycoprotein receptor activity; heparin binding; lipase binding; low-density lipoprotein particle receptor binding; receptor antagonist activity; unfolded protein binding; very-low-density lipoprotein particle receptor binding; |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionLRPAP1 may be a potential therapeutic target in diseases where its interactions with LRP1 play a crucial role, such as neurodegenerative disorders, cardiovascular diseases, and certain cancers.
LRPAP1 has been implicated in immune system regulation, and its interactions with LRP1 may impact immune responses, suggesting potential applications in immunotherapy.
Genetic variations or mutations in the LRPAP1 gene have been investigated in the context of certain diseases, and understanding these variations may provide insights into disease susceptibility and progression.
LRPAP1 plays a role in lipoprotein metabolism by influencing the function of LRP1, a receptor involved in the clearance of lipoproteins from the circulation, thus impacting cardiovascular health.
Understanding the role of LRPAP1 in various diseases may contribute to personalized medicine by identifying individuals who could benefit from targeted therapies based on their specific genetic or molecular profiles.
Customer Reviews (3)
Write a reviewThis technical support is invaluable, especially for researchers who may be new to working with LRPAP1 protein or if they encounter specific difficulties while conducting their experiments.
They can offer guidance on experimental design, protocol optimization, and troubleshooting, helping researchers overcome any challenges encountered during their trials.
the manufacturer's technical expertise can be advantageous to researchers.
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