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Recombinant Mouse SFTPD cell lysate

Cat.No. : SFTPD-2675MCL
Product Overview : Mouse SFTPD derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Sftpd surfactant associated protein D [ Mus musculus ]
Official Symbol : SFTPD
Synonyms : SFTPD; surfactant associated protein D; pulmonary surfactant-associated protein D; PSP-D; lung surfactant protein D; SP-D; Sftp4; AI573415;
Gene ID : 20390
mRNA Refseq : NM_009160
Protein Refseq : NP_033186
Pathway : Phagosome, organism-specific biosystem; Phagosome, conserved biosystem;
Function : bacterial cell surface binding; binding; identical protein binding; lipopolysaccharide binding; monosaccharide binding; sugar binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How is SFTPD involved in the regulation of inflammation? 02/14/2022

SFTPD regulates immune cell activity, helping to control inflammation in the lungs.

Are there known genetic variations in SFTPD associated with specific diseases? 09/04/2021

Certain genetic variations in SFTPD have been linked to increased susceptibility to respiratory diseases.

What is the primary function of SFTPD in the immune system? 08/15/2020

SFTPD enhances the clearance of pathogens and modulates immune responses.

What role does SFTPD play in respiratory infections? 06/15/2020

It binds to pathogens, aiding in their clearance and protecting against respiratory infections.

What therapeutic strategies are being explored that involve SFTPD? 03/30/2020

Therapies are being explored to modulate SFTPD levels in diseases like COPD and pulmonary fibrosis.

How does SFTPD contribute to pulmonary homeostasis? 01/01/2019

It maintains lung function by participating in surfactant homeostasis and immune regulation.

In which tissues is SFTPD predominantly expressed? 10/13/2018

SFTPD is primarily expressed in the lungs, especially in alveolar and bronchiolar epithelial cells.

Customer Reviews (3)

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Reviews
11/17/2022

    Quality product, fast delivery.

    04/21/2021

      Fast, efficient, and reliable.

      02/06/2018

        Excellent performance in assay.

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