Recombinant Human FLT4 cell lysate
Cat.No. : | FLT4-2709HCL |
Product Overview : | Human VEGFR3 / FLT4 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | FLT4 fms-related tyrosine kinase 4 [ Homo sapiens ] |
Official Symbol : | FLT4 |
Synonyms : | FLT4; fms-related tyrosine kinase 4; vascular endothelial growth factor receptor 3; PCL; VEGFR3; FLT-4; VEGFR-3; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; fms-like tyrosine kinase 4; tyrosine-protein kinase receptor FLT4; FLT41; LMPH1A; |
Gene ID : | 2324 |
mRNA Refseq : | NM_002020 |
Protein Refseq : | NP_002011 |
MIM : | 136352 |
UniProt ID : | P35916 |
Chromosome Location : | 5q34-q35 |
Pathway : | Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem; Signal Transduction, organism-specific biosystem; Signaling by VEGF, organism-specific biosystem; Signaling events mediated by VEGFR1 and VEGFR2, organism-specific biosystem; |
Function : | ATP binding; growth factor binding; nucleotide binding; protein binding; protein phosphatase binding; receptor activity; transmembrane receptor protein tyrosine kinase activity; vascular endothelial growth factor-activated receptor activity; vascular endothelial growth factor-activated receptor activity; |
Products Types
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FLT4-2055H | Recombinant Human FLT4 Protein, Fc/His-tagged | +Inquiry |
FLT4-4377H | Active Recombinant Human FLT4 Protein, GST/His-tagged | +Inquiry |
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◆ Lysates | ||
FLT4-2016MCL | Recombinant Mouse FLT4 cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-1594 | Flt4 Activity Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionFLT4 protein is associated with various tumor-related markers such as malignant lymphoma, breast cancer, and lung cancer.
FLT4 protein is a key regulator of lymphedema and plays an important role in lymphangiogenesis and lymphatic drainage.
Yes, mutations in the FLT4 protein can lead to congenital lymphedema, a condition caused by abnormal lymphatic vessel development.
The protein is considered an important target in anticancer therapy, and inhibition of its activity may reduce tumor angiogenesis and metastasis.
FLT4 protein plays an important role in pulmonary hypertension, and upregulation of its activity may lead to pathological changes such as vascular remodeling and pulmonary artery stenosis.
This is involved in the regulation of angiogenesis and lymphangiogenesis by binding to vascular endothelial growth factor C (VEGF-C) and vascular endothelial growth factor D (VEGF-D).
Customer Reviews (3)
Write a reviewThe catalytic activity of FLT4 is very strong, which can significantly improve the selectivity and yield of chemical reactions, and its application potential is great.
We were reassured that the quality of FLT4 was consistent.
Using FLT4 for experiments, I can focus more on experimental design and data analysis without worrying about the reproducibility of experimental results.
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