Recombinant Human FN1 cell lysate
Cat.No. : | FN1-2956HCL |
Product Overview : | Human Fibronectin / Fibronectin Fragment 2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | FN1 fibronectin 1 [ Homo sapiens ] |
Official Symbol : | FN1 |
Synonyms : | FN1; fibronectin 1; fibronectin; CIG; cold insoluble globulin; FINC; GFND2; LETS; migration stimulating factor; MSF; cold-insoluble globulin; migration-stimulating factor; FN; FNZ; ED-B; GFND; DKFZp686H0342; DKFZp686I1370; DKFZp686F10164; DKFZp686O13149; |
Gene ID : | 2335 |
mRNA Refseq : | NM_002026 |
Protein Refseq : | NP_002017 |
MIM : | 135600 |
UniProt ID : | P02751 |
Chromosome Location : | 2q34 |
Pathway : | Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; |
Function : | collagen binding; extracellular matrix structural constituent; heparin binding; protein binding; |
Products Types
◆ Recombinant Protein | ||
FN1-2959H | Recombinant Human FN1 Protein, His (Fc)-Avi-tagged | +Inquiry |
FN1-938H | Recombinant Human FN1 Protein, His-tagged | +Inquiry |
FN1-4400H | Recombinant Human FN1 Protein, GST-tagged | +Inquiry |
FN1-2741P | Recombinant Pig FN1 Protein, His-tagged | +Inquiry |
FN1-01H | Recombinant Human FN1 Protein, T7-His-TEV-tagged | +Inquiry |
◆ Native Protein | ||
Fn1-5424M | Native Mouse Fibronectin | +Inquiry |
FN1-4399H | Native Human FN1 Protein | +Inquiry |
FN1-701H | Native Human Fibronectin 1 | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionIt is expressed in a variety of organs and tissues, including skin, kidneys, cardiovascular system, liver, etc.
FN1 protein is involved in the migration, adhesion and activation of inflammatory cells during the inflammatory response, and plays an important role in the development of inflammation.
Yes, multiple factors have been found to regulate the expression and function of FN1 protein, including transcription factors, growth factors, etc.
FN1 protein is involved in the invasion and metastasis of tumor cells, which can provide matrix support and guide signals to promote the migration and invasion of tumor cells.
This protein is involved in key steps such as fibrosis, angiogenesis and interstitial cell proliferation in the wound healing process, and plays an important role in wound repair.
The protein plays an important role in key steps such as extracellular matrix remodeling, cell adhesion, and directed differentiation of mesenchymal stem cells during tissue repair.
Customer Reviews (3)
Write a reviewThe preservation conditions are wide and the efficiency is high.
Experimental process is convenient, which greatly improves the efficiency of scientific research.
Biological activity of this protein is amazing, and you can see that its production process is very pure, which has a crucial effect on the quality of the product.
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