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Recombinant Human FN1 cell lysate

Cat.No. : FN1-2956HCL
Product Overview : Human Fibronectin / Fibronectin Fragment 2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : FN1 fibronectin 1 [ Homo sapiens ]
Official Symbol : FN1
Synonyms : FN1; fibronectin 1; fibronectin; CIG; cold insoluble globulin; FINC; GFND2; LETS; migration stimulating factor; MSF; cold-insoluble globulin; migration-stimulating factor; FN; FNZ; ED-B; GFND; DKFZp686H0342; DKFZp686I1370; DKFZp686F10164; DKFZp686O13149;
Gene ID : 2335
mRNA Refseq : NM_002026
Protein Refseq : NP_002017
MIM : 135600
UniProt ID : P02751
Chromosome Location : 2q34
Pathway : Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem;
Function : collagen binding; extracellular matrix structural constituent; heparin binding; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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which organs and tissues is the FN1 protein expressed? 12/21/2019

It is expressed in a variety of organs and tissues, including skin, kidneys, cardiovascular system, liver, etc.

How about the FN1 protein in inflammatory diseases? 09/09/2019

FN1 protein is involved in the migration, adhesion and activation of inflammatory cells during the inflammatory response, and plays an important role in the development of inflammation.

Have regulators of the FN1 protein been identified? 07/16/2019

Yes, multiple factors have been found to regulate the expression and function of FN1 protein, including transcription factors, growth factors, etc.

What is the relationship between FN1 protein and tumor metastasis? 06/26/2019

FN1 protein is involved in the invasion and metastasis of tumor cells, which can provide matrix support and guide signals to promote the migration and invasion of tumor cells.

What is the role of FN1 protein in wound healing? 04/15/2019

This protein is involved in key steps such as fibrosis, angiogenesis and interstitial cell proliferation in the wound healing process, and plays an important role in wound repair.

How about the mechanism of action of FN1 protein in tissue repair? 04/13/2019

The protein plays an important role in key steps such as extracellular matrix remodeling, cell adhesion, and directed differentiation of mesenchymal stem cells during tissue repair.

Customer Reviews (3)

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Reviews
06/21/2021

    The preservation conditions are wide and the efficiency is high.

    08/14/2019

      Experimental process is convenient, which greatly improves the efficiency of scientific research.

      03/25/2019

        Biological activity of this protein is amazing, and you can see that its production process is very pure, which has a crucial effect on the quality of the product.

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