|Form:||The human amygdala tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.|
|Preparation method:||Human amygdala tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human amygdala tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the amygdala tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The amygdala tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each.|
|Safety Testing:||This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request.|
|Expiration Date:||One half year from date of receipt|
|Size:||0.1 mg, 0.5 mg|
|Storage Instruction:||This lysate should be aliquoted in working amounts and may be stored for up to one month at 2-8°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C.|
|Applications:||This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.|
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