Human Lymph Node Normal Tissue Lysate

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Human Lymph Node Normal Tissue Lysate

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Cat.No. : Lymph Node-32H
Species : Human
Form : The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Concentration : 2.0 mg/ml
Tissue Type : Lymph Node
Preparation method : Lymph Node tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation.
Safety Testing : This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request.
Diagnosis : Normal
Expiration Date : One year from date of receipt
Size : 0.1 mg, 0.5 mg
Storage Instruction : For short term storage of this tissue lysate, freeze in single use aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For long term storage freeze working aliquots at -70°C.
Applications : This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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