| Cat.No. : |
Small Intestine-59H
Optional Service: Optional requirements on this protein
|
| Species : |
Human |
| Form : |
The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
| Concentration : |
0.2 mg/ml |
| Tissue Type : |
Small Intestine |
| Preparation method : |
Small Intestine tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. |
| Safety Testing : |
This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request. |
| Diagnosis : |
Tumor |
| Expiration Date : |
One year from date of receipt |
| Size : |
0.1 mg, 0.5 mg |
| Storage Instruction : |
This lysate should be aliquoted in working amounts and may be stored for up to one month at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C. |
| Applications : |
This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.