| Cat.No. : |
Kidney-99M |
| Species : |
Mouse |
| Form : |
The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
| Concentration : |
2.0 mg/ml |
| Tissue Type : |
Kidney |
| Preparation method : |
Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. |
| Safety Testing : |
This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request. |
| Diagnosis : |
Normal |
| Expiration Date : |
One year from date of receipt |
| Size : |
0.1 mg, 0.5 mg |
| Storage Instruction : |
For short term storage of this tissue lysate, freeze in single use aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For long term storage freeze working aliquots at -70°C. |
| Applications : |
This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.