Mouse Kidney Tissue Lysate

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Cat.No. :	Kidney-99M
Species :	Mouse
Form :	The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Concentration :	2.0 mg/ml
Tissue Type :	Kidney
Preparation method :	Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
Safety Testing :	This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request.
Diagnosis :	Normal
Expiration Date :	One year from date of receipt
Size :	0.1 mg, 0.5 mg
Storage Instruction :	For short term storage of this tissue lysate, freeze in single use aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For long term storage freeze working aliquots at -70°C.
Applications :	This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.