Rat Cerebellum Tissue Lysate

• Cat.No.: Cerebellum-133R

Specification

• Species: Rat
• Tag: Non
• Form: The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
• Concentration: 2.0 mg/ml
• Tissue Type: Cerebellum
• Preparation method: Cerebellum tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
• Safety Testing: This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request.
• Diagnosis: Normal
• Expiration Date: One year from date of receipt
• Size: 0.1 mg, 0.5 mg
• Storage Instruction: This lysate should be aliquoted in working amounts and may be stored for up to one month at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C.
• Applications: This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.