3T3 Balb Whole Cell Lysate Balb-150M

Specification

Cat.No. :	Balb-150M
Species :	Mouse
Form :	The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Concentration :	2.0 mg/ml
Cell line :	BALB/3T3 clone A31 (Mouse Embryo)
Tissue Type :	Mouse Embryo
Growth media :	DMEM & 10% FBS (fetal bovine serum)
Preparation method :	3T3 (Balb) lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
Expiration Date :	One year from date of receipt
Size :	0.1 mg, 0.5 mg
Storage Instruction :	This lysate should be aliquoted in working amounts and may be stored for up to one month at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C.
Applications :	This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.

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