| Cat.No.: | Mouse Embryo-152M |
| Species: | Mouse |
| Form: | The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
| Concentration: | 2.0 mg/ml |
| Cell line: | NIH/3T3 (Embryo) |
| Tissue Type: | Mouse Embryo |
| Growth media: | DMEM & 10% FBS (Fetal Bovine Serum) |
| Preparation method: | 3T3 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. |
| Expiration Date: | One year from date of receipt |
| Size: | 0.1 mg, 0.5 mg |
| Storage Instruction: | This whole cell lysate is stable for three months at -20°C |
| Applications: | This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel. |
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