L1210 Whole Cell Lysate

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L1210 Whole Cell Lysate

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Cat.No. : L1210-168H
Species : Human
Form : The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Concentration : 2.0 mg/ml
Cell line : L1210 (Lymphocytic Leukemia)
Growth media : DMEM & 10% Horse Serum (Equine Serum)
Preparation method : L1210 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
Expiration Date : One year from date of receipt
Size : 0.1 mg, 0.5 mg
Storage Instruction : This lysate should be aliquoted in working amounts and may be stored for up to one month at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C.
Applications : This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.

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