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MDA-MB-361 Whole Cell Lysate

Cat.No. : MDA-MB-361-170H
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Species : Human
Form : The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Concentration : 2.0 mg/ml
Cell line : MDA-MB-361 (Human Breast Adenocarcinoma)
Growth media : DMEM/Hams F12 & 10% NCS (Newborn Calf Serum)
Preparation method : MDA-MB-361 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
Expiration Date : One year from date of receipt
Size : 0.1 mg, 0.5 mg
Storage Instruction : This whole cell lysate is stable for three months at -20°C
Applications : This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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