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| Cat.No.: | Brain-45P |
| Source: | Porcine brain tissue |
| Species: | Porcine |
| Concentration: | 2 mg/mL or better |
| Tissue Type: | Brain |
| Shipping: | Dry ice |
| Preparation method: | Porcine brain tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70?C. The porcine brain tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the brain tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The brain tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
| Diagnosis: | Normal |
| Storage Buffer: | HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40. |
| Storage Instruction: | Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt. |
| Applications: | Brain lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel. |
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