Porcine Brain Lysate Brain-45P

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Porcine Brain Lysate

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Cat.No. :	Brain-45P
Source :	Porcine brain tissue
Species :	Porcine
Concentration :	2 mg/mL or better
Tissue Type :	Brain
Shipping :	Dry ice
Preparation method :	Porcine brain tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70?C. The porcine brain tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the brain tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The brain tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Diagnosis :	Normal
Storage Buffer :	HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
Storage Instruction :	Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Applications :	Brain lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.