Expand Expand| Cat.No. : | Brain-45P |
| Source : | Porcine brain tissue |
| Species : | Porcine |
| Concentration : | 2 mg/mL or better |
| Tissue Type : | Brain |
| Shipping : | Dry ice |
| Preparation method : | Porcine brain tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70?C. The porcine brain tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the brain tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The brain tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
| Diagnosis : | Normal |
| Storage Buffer : | HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40. |
| Storage Instruction : | Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt. |
| Applications : | Brain lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel. |
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