Human Fetal Liver Membrane Lysate Fetal Liver-148H

Human Fetal Liver Membrane Lysate

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Human Fetal Liver Membrane Lysate

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Cat.No. : Fetal Liver-148H
Source : Fetal Human Liver tissue
Species : Human
Concentration : 2 mg/mL or better
Tissue Type : Liver
Shipping : Dry ice
Preparation method : Human liver tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human liver tissue was frozen in liquid nitrogen immediately after excision and then stored at -70?C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated liver tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated liver tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
Diagnosis : Normal
Storage Buffer : HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
Storage Instruction : Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Applications : Fetal Liver membrane protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.

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