Human Fetal Spleen Membrane Lysate

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Human Fetal Spleen Membrane Lysate

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Cat.No. : Fetal Spleen-167H
Source : Fetal Human Spleen tissue
Species : Human
Concentration : 2 mg/mL or better
Tissue Type : Spleen
Shipping : Dry ice
Preparation method : Human spleen tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human spleen tissue was frozen in liquid nitrogen immediately after excision and then stored at -70?C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated spleen tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated spleen tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
Diagnosis : Normal
Storage Buffer : HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
Storage Instruction : Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Applications : Fetal Spleen membrane protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.

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Fetal Spleen-166HHuman Fetal Spleen Lysate+Inquiry

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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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