Human Fetal Temporal Lobe Membrane Lysate Fetal Temporal Lobe -170H

Human Fetal Temporal Lobe Membrane Lysate

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Human Fetal Temporal Lobe Membrane Lysate

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Cat.No. : Fetal Temporal Lobe -170H
Source : Fetal Human Temporal Lobe tissue
Species : Human
Concentration : 2 mg/mL or better
Tissue Type : Brain: Temporal Lobe
Shipping : Dry ice
Preparation method : Human brain temporal lobe tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human temporal lobe tissue was frozen in liquid nitrogen immediately after excision and then stored at -70?C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated brain temporal lobe tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated brain temporal lobe tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
Diagnosis : Normal
Storage Buffer : HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
Storage Instruction : Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Applications : Fetal Temporal Lobe membrane protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.

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