| Cat.No. : |
Ileum-244H
Optional Service: Optional requirements on this protein
|
| Source : |
Human Small intestine: Ileum tissue |
| Species : |
Human |
| Concentration : |
2 mg/mL or better |
| Tissue Type : |
Small intestine: Ileum |
| Shipping : |
Dry ice |
| Preparation method : |
Human small intestine: Ileum tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70?C. The human small intestine: Ileum tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the small intestine: Ileum tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The small intestine: Ileum tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
| Diagnosis : |
Diseased |
| Storage Buffer : |
HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40. |
| Storage Instruction : |
Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt. |
| Applications : |
Small intestine: Ileum lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.