Human Ileum Membrane Lysate
Cat.No. : | Ileum-248H |
- Specification
- Gene Information
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Source : | Human Small intestine: Ileum tissue |
Species : | Human |
Concentration : | 2 mg/mL or better |
Tissue Type : | Small intestine: Ileum |
Shipping : | Dry ice |
Preparation method : | Human small intestine: Ileum tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human small intestine: Ileum tissue was frozen in liquid nitrogen immediately after excision and then stored at -70?C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine: Ileum tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine: Ileum tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
Diagnosis : | Normal |
Storage Buffer : | HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40. |
Storage Instruction : | Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt. |
Applications : | Small intestine: Ileum membrane protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel. |
Products Types
◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionThe Ileum the importance in the intestinal flora on the absorption of nutrients and the influence of the body's immune system, etc. The change of the microbial groups can cause bad influence to Ileum function, so as to induce different intestinal disease.
The Ileum and colon microbial components difference is very big, in the Ileum flora more diversified, while the colon is relatively single.
Ileum intestinal flora and digestion, immune system and so on are closely related. Intestinal flora on the immune function of play Ileum absorb and plays an important role, such as the absorption of the synthesis of vitamin K and B vitamins, etc.
Compared to other parts of the gut, bulging Ileum mucosa more stereo, help to increase the absorption area. In addition, Ileum and one of the main accumulation of B cells and T cells.
This protein inflammation of the main causes include infection, intestinal flora disturbance, etc. Mechanism, the inflammatory response is composed of a series of immune cells, molecules and reaction, cause inflammation reaction mechanism including immune mediated, cytokine mediated or other factors.
Ileum of the intestinal immune cell types including Th17, Th1, Th2, Treg, distributed in the mucosa and lymphoid tissue, and to such factors as the intestinal flora and digestive there are complex interactions.
Customer Reviews (3)
Write a reviewThe potency and power of this protein met my expectations.
No side effects, high safety.
Strong ability to penetrate tissue, can quickly reach the target.
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