Human K 562 Cytoplasmic Lysate

• Cat.No.: K 562-257H

Specification

• Species: Human
• Source: K562
• Tag: Non
• Concentration: 2 mg/mL or better
• Cell line: K 562 (Human Chronic Myelogenous Leukemia; Bone Marrow)
• Shipping: Dry ice
• Preparation method: K 562 (Human Chronic Myelogenous Leukemia; Bone Marrow) Cell cytoplasmic Protein Lysate cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated K 562 (Human Chronic Myelogenous Leukemia; Bone Marrow) cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated K 562 (Human Chronic Myelogenous Leukemia; Bone Marrow) tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.
• Diagnosis: Tumor
• Storage Buffer: HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
• Storage Instruction: Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
• Applications: K 562 (Chronic Myelogenous Leukemia; Bone Marrow) Cell Cytoplasmic protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.