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Human Liver Lysate
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Cat.No. : | Liver-290H Optional Service: Optional requirements on this protein |
Source : | Human liver tissue |
Species : | Human |
Concentration : | 2 mg/mL |
Tissue Type : | Liver |
Shipping : | Dry ice |
Preparation method : | Liver tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Diagnosis : | Normal |
Storage Buffer : | Liver lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol. |
Storage Instruction : | Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt. |
◆ Lysates
Liver-28H | Human Liver Tissue Lysate | +Inquiry |
Liver-283H | Human Liver Left Lobe Lupus Lysate | +Inquiry |
Liver-282H | Human Liver Cytoplasmic Lysate | +Inquiry |
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