Human Skin Liver Cirrhosis Lysate Skin-440H

Human Skin Liver Cirrhosis Lysate

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Human Skin Liver Cirrhosis Lysate

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Cat.No. : Skin-440H
Source : Human Skin tissue
Species : Human
Concentration : 2 mg/mL or better
Tissue Type : Skin
Shipping : Dry ice
Preparation method : Human skin tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70?C. The human skin tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skin tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skin tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Diagnosis : Diseased
Storage Buffer : HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
Storage Instruction : Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Applications : Skin lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.

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