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| Cat.No.: | Uterus-549H |
| Source: | Human Uterus tissue |
| Species: | Human |
| Concentration: | 2 mg/mL or better |
| Tissue Type: | Uterus |
| Shipping: | Dry ice |
| Preparation method: | Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70?C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
| Diagnosis: | Normal |
| Storage Buffer: | HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40. |
| Storage Instruction: | Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt. |
| Applications: | Uterus membrane protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel. |
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