Recombinant Human PARP14, His-GST-tagged

• Cat.No.: PARP14-36H
• Product Overview: Recombinant Human PARP14 (1470–1801), fused with N-terminal His-GST-tag, was expressed in a Baculovirus infected Sf9 cell expression system.

Specification

• Description: Poly(ADP-ribosyl)ation is an immediate DNA damage-dependent posttranslational modification of histones and other nuclear proteins that contributes to the survival of injured proliferating cells. PARP14 belongs to the superfamily of enzymes that perform this modification.
• Source: Sf9 insect cells
• Species: Human
• Tag: His-GST
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 188 mM imidazole, 0.04% Tween-20, 3 mM DTT, 20% glycerol.
• Molecular Mass: 66.5 kDa
• Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: >6 months at -80°C.

Gene Information

• Gene Name: PARP14 poly (ADP-ribose) polymerase family, member 14 [ Homo sapiens (human) ]
• Official Symbol: PARP14
• Synonyms: PARP14; poly (ADP-ribose) polymerase family, member 14; poly [ADP-ribose] polymerase 14; KIAA1268; pART8; B aggressive lymphoma protein 2; BAL2; Collaborator of STAT6; KIAA1268; PARP 14; pART8; Poly (ADP ribose) polymerase family member 14; collaborator of STAT6; B-aggressive lymphoma 2; b aggressive lymphoma protein 2; BAL2; PARP-14
• Gene ID: 54625
• mRNA Refseq: NM_017554
• Protein Refseq: NP_060024
• MIM: 610028
• UniProt ID: Q460N5
• Chromosome Location: 3q21.1
• Pathway: IL4-mediated signaling events
• Function: NAD+ ADP-ribosyltransferase activity