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Recombinant Human ZFP36L1 293 Cell Lysate

Cat.No.: ZFP36L1-180HCL
Description: Antigen standard for zinc finger protein 36, C3H type-like 1 (ZFP36L1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ZFP36L1 zinc finger protein 36, C3H type-like 1 [ Homo sapiens ]
Official Symbol: ZFP36L1
Synonyms: ZFP36L1; zinc finger protein 36, C3H type-like 1; BRF1, zinc finger protein, C3H type, 36 like 1; zinc finger protein 36, C3H1 type-like 1; Berg36; cMG1; ERF1; RNF162B; TIS11B; EGF-response factor 1; butyrate response factor 1; early response factor Berg36; zinc finger protein, C3H type, 36-like 1; BRF1; ERF-1;
Gene ID: 677
mRNA Refseq: NM_001244698
Protein Refseq: NP_001231627
MIM: 601064
UniProt ID: Q07352
Chromosome Location: 14q22-q24
Pathway: Destabilization of mRNA by Butyrate Response Factor 1 (BRF1), organism-specific biosystem; Gene Expression, organism-specific biosystem; Regulation of mRNA Stability by Proteins that Bind AU-rich Elements, organism-specific biosystem; Validated targets of C-MYC transcriptional repression, organism-specific biosystem;

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