"YY1AP1" Related Products

Recombinant Human YY1AP1 293 Cell Lysate

Cat.No.: YY1AP1-227HCL
Description: Antigen standard for YY1 associated protein 1 (YY1AP1), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: YY1AP1 YY1 associated protein 1 [ Homo sapiens ]
Official Symbol: YY1AP1
Synonyms: YY1AP1; YY1 associated protein 1; YY1-associated protein 1; HCCA2; YAP; YY1AP; hepatocellular carcinoma-associated protein 2; hepatocellular carcinoma susceptibility protein; HCCA1; FLJ10875; FLJ13914;
Gene ID: 55249
mRNA Refseq: NM_001198899
Protein Refseq: NP_001185828
MIM: 607860
UniProt ID: Q9H869
Chromosome Location: 1q22

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