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Recombinant Human UQCRB 293 Cell Lysate

Cat.No.: UQCRB-489HCL
Description: Antigen standard for ubiquinol-cytochrome c reductase binding protein (UQCRB), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: UQCRB ubiquinol-cytochrome c reductase binding protein [ Homo sapiens (human) ]
Official Symbol: UQCRB
Synonyms: UQCRB; QPC; QCR7; QP-C; UQBC; UQBP; UQPC; UQCR6; MC3DN3
Gene ID: 7381
mRNA Refseq: NM_006294
Protein Refseq: NP_006285
UniProt ID: P14927
Chromosome Location: 8q22
Pathway: Alzheimer's disease, organism-specific and conserved biosystem; Cardiac muscle contraction, organism-specific and conserved biosystem

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