Recombinant Human UFD1L 293 Cell Lysate

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Recombinant Human UFD1L 293 Cell Lysate

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Cat.No. : UFD1L-520HCL
Description : Antigen standard for ubiquitin fusion degradation 1 like (yeast) (UFD1L), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : UFD1L ubiquitin fusion degradation 1 like (yeast) [ Homo sapiens ]
Official Symbol : UFD1L
Synonyms : UFD1L; ubiquitin fusion degradation 1 like (yeast); ubiquitin fusion degradation 1 like; ubiquitin fusion degradation protein 1 homolog; UFD1; UB fusion protein 1;
Gene ID : 7353
mRNA Refseq : NM_001035247
Protein Refseq : NP_001030324
MIM : 601754
UniProt ID : Q92890
Chromosome Location : 22q11.2
Pathway : HRD1/SEL1 ERAD complex, organism-specific biosystem; HRD1/SEL1 ERAD complex, conserved biosystem; Protein processing in endoplasmic reticulum, organism-specific biosystem; Protein processing in endoplasmic reticulum, conserved biosystem; p97-Ufd1-Npl4 complex, organism-specific biosystem;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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