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Recombinant Human TUBB4 293 Cell Lysate

Cat.No. : TUBB4A-647HCL
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  • Gene Information
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Description : Antigen standard for tubulin, beta 4 (TUBB4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : TUBB4A tubulin, beta 4A class IVa [ Homo sapiens (human) ]
Official Symbol : TUBB4A
Synonyms : TUBB4; DYT4; TUBB4; TUBB5; beta-5
Gene ID : 10382
mRNA Refseq : NM_006087
Protein Refseq : NP_006078
UniProt ID : P04350
Chromosome Location : 19p13.3
Pathway : Cell Cycle, organism-specific biosystem; Centrosome maturation, organism-specific biosystem; Formation of tubulin folding intermediates by CCT/TriC, organism-specific biosystem

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What potential therapeutic applications arise from targeting TUBB4A in neurodegenerative diseases? 06/08/2023

Targeting TUBB4A could offer new treatments for neurodegenerative conditions by stabilizing neuronal structure.

What is the primary role of TUBB4A in cellular structure? 03/26/2022

TUBB4A, a tubulin isoform, is crucial for maintaining the cytoskeleton and cellular shape.

What role does TUBB4A play in neurological function? 10/01/2021

TUBB4A is important for brain function, involved in neuronal structure and signaling.

How does TUBB4A contribute to microtubule formation? 04/28/2021

It plays a key role in assembling microtubules, essential for cell division and intracellular transport.

How does TUBB4A interact with other cytoskeletal proteins? 02/18/2021

TUBB4A works with other cytoskeletal proteins to maintain cell structure and facilitate movement.

How do genetic variations in TUBB4A affect neurologic disorders? 12/29/2019

Mutations in TUBB4A can lead to a range of neurological conditions, affecting brain development and function.

What is the impact of altered TUBB4A activity on cellular dynamics? 05/04/2017

Altered TUBB4A activity can disrupt microtubule dynamics, impacting cell function and stability.

Customer Reviews (3)

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Reviews
03/20/2019

    Key contributor to discoveries, enhances our insights.

    10/05/2018

      Reliable data source, supports our research consistently.

      03/23/2017

        Dependable partner, a vital research resource.

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