Recombinant Human TUBB4 293 Cell Lysate
Cat.No. : | TUBB4A-647HCL |
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- Gene Information
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Description : | Antigen standard for tubulin, beta 4 (TUBB4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | TUBB4A tubulin, beta 4A class IVa [ Homo sapiens (human) ] |
Official Symbol : | TUBB4A |
Synonyms : | TUBB4; DYT4; TUBB4; TUBB5; beta-5 |
Gene ID : | 10382 |
mRNA Refseq : | NM_006087 |
Protein Refseq : | NP_006078 |
UniProt ID : | P04350 |
Chromosome Location : | 19p13.3 |
Pathway : | Cell Cycle, organism-specific biosystem; Centrosome maturation, organism-specific biosystem; Formation of tubulin folding intermediates by CCT/TriC, organism-specific biosystem |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionTargeting TUBB4A could offer new treatments for neurodegenerative conditions by stabilizing neuronal structure.
TUBB4A, a tubulin isoform, is crucial for maintaining the cytoskeleton and cellular shape.
TUBB4A is important for brain function, involved in neuronal structure and signaling.
It plays a key role in assembling microtubules, essential for cell division and intracellular transport.
TUBB4A works with other cytoskeletal proteins to maintain cell structure and facilitate movement.
Mutations in TUBB4A can lead to a range of neurological conditions, affecting brain development and function.
Altered TUBB4A activity can disrupt microtubule dynamics, impacting cell function and stability.
Customer Reviews (3)
Write a reviewKey contributor to discoveries, enhances our insights.
Reliable data source, supports our research consistently.
Dependable partner, a vital research resource.
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